This is an exciting opportunity for an enthusiastic biochemist with experience with molecular biology, protein biochemistry, and ideally antibody libraries (or similar) and display techniques to join the ADDovenom team, which is developing novel antivenoms to treat snakebite envenoming.
Using state-of-the-art protein engineering and display technology, you will generate high-affinity binders against diverse toxins from snake venoms. These will be selected to neutralise the pathogenic function of toxins, for their use as antivenom. You will use a nanobody library already available in the lab and also newly designed library to select high-affinity binders against different toxin-targets using Ribosome Display. Professor Christiane Berger-Schaffitzel has been part of the team developing Ribosome Display as an in vitro selection and evolution method to generate high-affinity antibodies from naïve libraries. Ribosome Display is established in the lab and currently used to select binders against diverse antigens for structural biology and biotechnological applications. Selected binders will be biophysically and structurally characterised in the Berger Schaffitzel laboratory; and tested for toxin neutralisation in enzymatic and cell-based assays and a murine model in Bristol and our collaborator’s lab at Liverpool School of Tropical Medicine.
For more information about the role, and details of how to apply, visit the University of Bristol website. The closing date for applications is 9 January 2023.